Journal:

Article Title: Actions of brain-derived neurotrophic factor on evoked and spontaneous EPSCs dissociate with maturation of neurones cultured from rat visual cortex

doi: 10.1111/j.1469-7793.2000.t01-1-00579.x

Figure Lengend Snippet: A, to the left is shown the time course of the frequency of mEPSCs. The frequency was measured every 30 s. BDNF and K252a were applied to this cell at 200 ng ml−1 and 200 nm, respectively, during the periods indicated by horizontal bars. To the right are shown cumulative plots of the amplitude distributions of mEPSCs 2–5 min before (continuous line) and 17–20 min after (dotted line) the BDNF application. Bin width is 2 pA. The total number of events before and after the BDNF application is 572 and 587, respectively. Other conventions are the same as in Fig. 1D. B, to the left is shown the time course of the frequency of mEPSCs. BDNF and anti-BDNF antibody were applied to this cell at 200 ng ml−1 and 5 μg ml−1, respectively, during the periods indicated by the horizontal bars. To the right are shown cumulative plots of the amplitude distributions of mEPSCs 2–5 min before (continuous line) and 17–20 min after (dotted line) the BDNF application. The total number of events is 452 and 464, respectively. Other conventions are the same as in Fig. 1D.

Article Snippet: Application of BDNF and drugs In most of the experiments, recombinant human BDNF (provided from Sumitomo Pharmaceutical Co., Ltd, Japan), or K252a (Kyowa Hakko Co., Ltd, Japan) was applied to neurones through the perfusion medium 10–15 min after the initiation of recordings.

Techniques:

Journal:

Article Title: Actions of brain-derived neurotrophic factor on evoked and spontaneous EPSCs dissociate with maturation of neurones cultured from rat visual cortex

doi: 10.1111/j.1469-7793.2000.t01-1-00579.x

Figure Lengend Snippet: Ratio of the frequency of mEPSCs during the application of BDNF alone, BDNF + K252a and BDNF + anti-BDNF antibody to the respective control value at the time points shown on the abscissa. Filled symbols with vertical bars represent means and twice the s.d. for the same group of cells.

Article Snippet: Application of BDNF and drugs In most of the experiments, recombinant human BDNF (provided from Sumitomo Pharmaceutical Co., Ltd, Japan), or K252a (Kyowa Hakko Co., Ltd, Japan) was applied to neurones through the perfusion medium 10–15 min after the initiation of recordings.

Techniques: